![]() ![]() Compatible with chemiluminescent substrates and fluorescent secondary antibodies. Western blotting: detection of the two unstained bands via the detection method used for the target protein.Western blot confirmation-detect the two unstained proteins (30 kDa and 80 kDa) using the detection system for the target protein Invitrogen SeeBlue Plus2 Pre-stained Protein Standard.SeeBlue Plus2 prestained protein standard (Life Technologies, Carlsbad, CA, United. available protein standards in predicting accurate protein molecular weights. Fluorescent visualization-detect the 10 stained proteins using the 670 nm red laser or 700 nm channel of a fluorescence imager protein concentration was quantitated by the.The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › SeeBlue Pre-Stained Standard consists of nine polypeptides that resolve into sharp, tight blue bands in the range of range of 4200 kDa allowing you to easily monitor protein electrophoresis and western transfer efficiency. transblot system (Biorad) and hybridized with antibodies to GCK (Santa Cruz) and. IBright Prestained Protein Ladder contains twelve recombinant proteins, ten (11 to 250 kDa) that are blue-stained and fluor-labeled for direct and near-IR fluorescent visualization and protein sizing, and two (30 kDa and 80 kDa) that are unstained and contain IgG binding sites to bind the primary and secondary antibodies used for chemiluminescent and fluorescent detection of the target protein. SeeBlue (Invitrogen) protein ladder is included to allow molecular weights. ![]()
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